Recombinant adeno-associated virus expressing the receptor-binding domain of severe acute respiratory syndrome coronavirus S protein elicits neutralizing antibodies: Implication for developing SARS vaccines.
Identifieur interne : 003D50 ( Main/Exploration ); précédent : 003D49; suivant : 003D51Recombinant adeno-associated virus expressing the receptor-binding domain of severe acute respiratory syndrome coronavirus S protein elicits neutralizing antibodies: Implication for developing SARS vaccines.
Auteurs : Lanying Du [République populaire de Chine] ; Yuxian He ; Yijia Wang ; Haojie Zhang ; Selene Ma ; Charlotte K L. Wong ; Sharon H W. Wu ; Fai Ng ; Jian-Dong Huang ; Kwok-Yung Yuen ; Shibo Jiang ; Yusen Zhou ; Bo-Jian ZhengSource :
- Virology [ 0042-6822 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (biosynthèse), Cellules HeLa, Dependovirus (génétique), Dependovirus (métabolisme), Femelle, Humains, Lignée cellulaire, Protéines recombinantes (métabolisme), Récepteurs viraux (métabolisme), Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Tests de neutralisation, Vaccins antiviraux (immunologie), Vecteurs génétiques, Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Anticorps antiviraux.
- génétique : Dependovirus.
- immunologie : Vaccins antiviraux, Virus du SRAS.
- métabolisme : Dependovirus, Protéines recombinantes, Récepteurs viraux.
- Animaux, Cellules HeLa, Femelle, Humains, Lignée cellulaire, Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Tests de neutralisation, Vecteurs génétiques.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (biosynthesis), Cell Line, Dependovirus (genetics), Dependovirus (metabolism), Female, Genetic Vectors, HeLa Cells, Humans, Mice, Mice, Inbred BALB C, Neutralization Tests, Protein Structure, Tertiary, Receptors, Virus (metabolism), Recombinant Proteins (metabolism), SARS Virus (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , biosynthesis : Antibodies, Viral.
- genetics : Dependovirus.
- immunology : SARS Virus, Viral Vaccines.
- metabolism : Dependovirus, Receptors, Virus, Recombinant Proteins.
- Animals, Cell Line, Female, Genetic Vectors, HeLa Cells, Humans, Mice, Mice, Inbred BALB C, Neutralization Tests, Protein Structure, Tertiary.
Abstract
Development of an effective vaccine for severe acute respiratory syndrome (SARS) remains to be a priority to prevent possible re-emergence of SARS coronavirus (SARS-CoV). We previously demonstrated that the receptor-binding domain (RBD) of SARS-CoV S protein is a major target of neutralizing antibodies. This suggests that the RBD may serve as an ideal vaccine candidate. Recombinant adeno-associated virus (rAAV) has been proven to be an effective system for gene delivery and vaccine development. In this study, a novel vaccine against SARS-CoV was developed based on the rAAV delivery system. The gene encoding RBD was cloned into a pAAV-IRES-hrGFP plasmid. The immunogenicity induced by the resulting recombinant RBD-rAAV was evaluated in BALB/c mice. The results demonstrated that (1) a single dose of RBD-rAAV vaccination could induce sufficient neutralizing antibody against SARS-CoV infection; (2) two more repeated doses of the vaccination boosted the neutralizing antibody to about 5 times of the level achieved by a single dose of the immunization and (3) the level of the antibody continued to increase for the entire duration of the experiment of 5.5 months. These results suggested that RBD-rAAV is a promising SARS candidate vaccine.
DOI: 10.1016/j.virol.2006.03.049
PubMed: 16793110
Affiliations:
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Wong</name></author><author><name sortKey="Wu, Sharon H W" sort="Wu, Sharon H W" uniqKey="Wu S" first="Sharon H W" last="Wu">Sharon H W. Wu</name></author><author><name sortKey="Ng, Fai" sort="Ng, Fai" uniqKey="Ng F" first="Fai" last="Ng">Fai Ng</name></author><author><name sortKey="Huang, Jian Dong" sort="Huang, Jian Dong" uniqKey="Huang J" first="Jian-Dong" last="Huang">Jian-Dong Huang</name></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></author><author><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name></author><author><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></author><author><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name></author></analytic><series><title level="j">Virology</title><idno type="ISSN">0042-6822</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Cell Line</term><term>Dependovirus (genetics)</term><term>Dependovirus (metabolism)</term><term>Female</term><term>Genetic Vectors</term><term>HeLa Cells</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Protein Structure, Tertiary</term><term>Receptors, Virus (metabolism)</term><term>Recombinant Proteins (metabolism)</term><term>SARS Virus (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (biosynthèse)</term><term>Cellules HeLa</term><term>Dependovirus (génétique)</term><term>Dependovirus (métabolisme)</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Protéines recombinantes (métabolisme)</term><term>Récepteurs viraux (métabolisme)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structure tertiaire des protéines</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (immunologie)</term><term>Vecteurs génétiques</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Dependovirus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Dependovirus</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Dependovirus</term><term>Receptors, Virus</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dependovirus</term><term>Protéines recombinantes</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Female</term><term>Genetic Vectors</term><term>HeLa Cells</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Neutralization Tests</term><term>Protein Structure, Tertiary</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules HeLa</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Structure tertiaire des protéines</term><term>Tests de neutralisation</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Development of an effective vaccine for severe acute respiratory syndrome (SARS) remains to be a priority to prevent possible re-emergence of SARS coronavirus (SARS-CoV). We previously demonstrated that the receptor-binding domain (RBD) of SARS-CoV S protein is a major target of neutralizing antibodies. This suggests that the RBD may serve as an ideal vaccine candidate. Recombinant adeno-associated virus (rAAV) has been proven to be an effective system for gene delivery and vaccine development. In this study, a novel vaccine against SARS-CoV was developed based on the rAAV delivery system. The gene encoding RBD was cloned into a pAAV-IRES-hrGFP plasmid. The immunogenicity induced by the resulting recombinant RBD-rAAV was evaluated in BALB/c mice. The results demonstrated that (1) a single dose of RBD-rAAV vaccination could induce sufficient neutralizing antibody against SARS-CoV infection; (2) two more repeated doses of the vaccination boosted the neutralizing antibody to about 5 times of the level achieved by a single dose of the immunization and (3) the level of the antibody continued to increase for the entire duration of the experiment of 5.5 months. These results suggested that RBD-rAAV is a promising SARS candidate vaccine.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="He, Yuxian" sort="He, Yuxian" uniqKey="He Y" first="Yuxian" last="He">Yuxian He</name><name sortKey="Huang, Jian Dong" sort="Huang, Jian Dong" uniqKey="Huang J" first="Jian-Dong" last="Huang">Jian-Dong Huang</name><name sortKey="Jiang, Shibo" sort="Jiang, Shibo" uniqKey="Jiang S" first="Shibo" last="Jiang">Shibo Jiang</name><name sortKey="Ma, Selene" sort="Ma, Selene" uniqKey="Ma S" first="Selene" last="Ma">Selene Ma</name><name sortKey="Ng, Fai" sort="Ng, Fai" uniqKey="Ng F" first="Fai" last="Ng">Fai Ng</name><name sortKey="Wang, Yijia" sort="Wang, Yijia" uniqKey="Wang Y" first="Yijia" last="Wang">Yijia Wang</name><name sortKey="Wong, Charlotte K L" sort="Wong, Charlotte K L" uniqKey="Wong C" first="Charlotte K L" last="Wong">Charlotte K L. Wong</name><name sortKey="Wu, Sharon H W" sort="Wu, Sharon H W" uniqKey="Wu S" first="Sharon H W" last="Wu">Sharon H W. Wu</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><name sortKey="Zhang, Haojie" sort="Zhang, Haojie" uniqKey="Zhang H" first="Haojie" last="Zhang">Haojie Zhang</name><name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name><name sortKey="Zhou, Yusen" sort="Zhou, Yusen" uniqKey="Zhou Y" first="Yusen" last="Zhou">Yusen Zhou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Du, Lanying" sort="Du, Lanying" uniqKey="Du L" first="Lanying" last="Du">Lanying Du</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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